2022

1. Hideo Nakano: High-Throughput Screening System of Monoclonal Antibodies from Single Rabbit and Human B Cells Using a Cell-Free Protein Synthesis System. The Korean Society for Biotechnology and Bioengineering Spring Meeting and International Symposium, April 14, 2022, online. [Invited Lecture]

2. Teruyo Ojima-Kato, Yuma Nishikawa, Yuki Furukawa, Hideo Nakano: The MSKIK Peptide Tag Releases secM-Mediated Translational Arrest. Summer Online Seminar 2022 for Young Researchers of the Society for Biotechnology, Japan, May 2022.

3. Hideo Nakano, Monami Kihara, Teruyo Ojima-Kato, Takaaki Kojima, Hiroshi Ueda, Tetsunari Hase, Makoto Ishii: Isolation and Characterization of Anti-SARS-CoV-2 Spike Human Monoclonal Antibodies from Single B Cells Derived from COVID-19 Patients Using a Cell-Free Protein Synthesis System. Autumn Meeting of the Society of Chemical Engineers, Japan, Biotechnology Division Symposium “Biochemical Engineering toward the Post-COVID Era,” September 16, 2022.

4. Beixi Jia, Takaaki Kojima, Teruyo Ojima-Kato, Hideo Nakano: Epitope Mapping of Fab Antibodies Synthesized in a Cell-Free System Using Ribosome Display and NGS. 193rd Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2022.

5. Daffa Sean Adinegoro, Hideo Nakano: Rapid Screening of Monoclonal Antibodies against G Protein-Coupled Receptors: Case Studies with Kisspeptin Receptor (GPR54). 15th Asian Congress on Biotechnology in Conjunction with the 7th International Symposium on Biomedical Engineering, Bali, Indonesia, October 2–6, 2022.

6. Rio Okuda, Rena Ito, Takaaki Kojima, Teruyo Ojima-Kato, Hideo Nakano: Development of an Antibody Affinity-Maturation Technology Using Ribosome Display with a Reconstituted Escherichia coli Cell-Free Protein Synthesis System. 193rd Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2022.

7. T.I.K. Munaweera, Jasmina Damnjanovic, Moeri Nezu, Maurizio Camagna, Takaaki Kojima, Kiyotaka Hitomi, Hideo Nakano, Naoto Nemoto: Extensive Analysis of Transglutaminase 1 Substrate Preferences Using cDNA Display. 193rd Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2022.

8. Yuma Nishikawa, Yuki Furukawa, Takaaki Kojima, Teruyo Ojima-Kato, Hideo Nakano: Study of the Mechanism by Which Peptides Enhance Protein Production. 193rd Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2022.

9. Teruyo Ojima-Kato, Yuma Nishikawa, Yuki Furukawa, Hideo Nakano: Release and Prevention of Translational Arrest by the MSKIK Peptide Tag. 74th Annual Meeting of the Society for Biotechnology, Japan, online, October 2022.

10. Beixi Jia, Takaaki Kojima, Teruyo Ojima-Kato, Hideo Nakano: Development of an Epitope-Mapping Technology Using PURE Ribosome Display. 74th Annual Meeting of the Society for Biotechnology, Japan, online, October 2022.

11. Aya Saito, Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Soluble Expression of Plasmanylethanolamine Desaturase. 74th Annual Meeting of the Society for Biotechnology, Japan, online, October 2022.

12. T.I.K. Munaweera, Jasmina Damnjanovic, Moeri Nezu, Maurizio Camagna, Takaaki Kojima, Kiyotaka Hitomi, Hideo Nakano, Naoto Nemoto: Extensive Analysis of Transglutaminase 1 Substrate Preferences Using cDNA Display. 74th Annual Meeting of the Society for Biotechnology, Japan, online, October 2022.

13. Hideo Nakano, Teruyo Ojima-Kato: Applications of Cell-Free Protein Synthesis for Generation and Characterization of Monoclonal Antibodies: A Versatile Tool for Nanomedicine. 74th Annual Meeting of the Society for Biotechnology, Japan, KSBB-BEST-SBJ Joint Symposium “Current Advances in Nanobiotechnology and Nanomedicine,” October 19, 2022. [Invited Lecture]

14. Jasmina Damnjanovic: Work-Life Balance in Academia and Productivity as a Researcher. 2022 Gender Equality Symposium, online, October 2022.

15. Jasmina Damnjanovic: Protein Engineering and Studies on Structure-Function Relationships of Enzymes. International Joint Workshop AC21, Nagoya University, Gifu University, and North Carolina State University, Nagoya, November 2022.

16. Yuma Nishikawa, Yuki Furukawa, Takaaki Kojima, Teruyo Ojima-Kato, Hideo Nakano: Study of the Mechanism by Which Peptides Enhance Protein Production. 2nd Amano Enzyme Research Grant Report Meeting, Nagoya, November 2022.

17. T.I.K. Munaweera, Jasmina Damnjanovic, Maurizio Camagna, Kiyotaka Hitomi, Naoto Nemoto, Hideo Nakano: Integrating cDNA Display and Bioinformatics to Reveal Substrate Preferences of Transglutaminase 1. 2nd Amano Enzyme Research Grant Report Meeting, Nagoya, November 2022.

18. Masaki Itaya, Boqing Liu, Teruyo Ojima-Kato, Hideo Nakano: Cell-Free Protein Synthesis of Antibody-Enzyme Fusion Proteins and Its Applications. 2nd Amano Enzyme Research Grant Report Meeting, Nagoya, November 2022.

19. Nana Odake, Jasmina Damnjanovic, Takaaki Kojima, Naoto Nemoto, Kiyotaka Hitomi, Maurizio Camagna, Hideo Nakano: Development of a Platform for Comprehensive Analysis of Transglutaminase Substrate Specificity by cDNA Display Using Next-Generation Sequencing and Bioinformatics. 2022 Transglutaminase Research Meeting, Nagoya, November 2022.

2021

1. Jasmina Damnjanovic, Nana Odake, Takaaki Kojima, Naoto Nemoto, Kiyotaka Hitomi, Hideo Nakano: cDNA Display Coupled with Next-Generation Sequencing for Activity-Based Selection: Comprehensive Analysis of Transglutaminase Substrate Preference. 2021 Sakura-Bio Meeting, May 2021.

2. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Prediction of Binding Motif Function Based on DNA Shape Features Extracted from Transcription Factor AoXlnR-Binding Sites. 2021 Sakura-Bio Meeting, May 2021.

3. Beixi Jia, Takaaki Kojima, Hideo Nakano: Rapid Tuning of Peptide Binding by PURE Ribosome Display and NGS. 2021 Sakura-Bio Meeting, May 2021.

4. Monami Kihara, Daffa Sean Adinegoro, Takaaki Kojima, Hideo Nakano: Development of a Method for Obtaining Rabbit Monoclonal Antibodies Using DNA Immunization. 2021 Sakura-Bio Meeting, May 2021.

5. Monami Kihara, Daffa Sean Adinegoro, Takaaki Kojima, Hideo Nakano: Development of a Method for Obtaining Rabbit Monoclonal Antibodies Using DNA Immunization. Summer Online Seminar 2021 for Young Researchers of the Society for Biotechnology, Japan, July 2021.

6. Rio Okuda, Rena Ito, Takaaki Kojima, Hideo Nakano: Development of an Antibody Affinity-Maturation Technology Using Ribosome Display with a Reconstituted Escherichia coli Cell-Free Protein Synthesis System. Summer Online Seminar 2021 for Young Researchers of the Society for Biotechnology, Japan, July 2021.

7. Beixi Jia, Takaaki Kojima, Hideo Nakano: Rapid Tuning of Peptide Binding by PURE Ribosome Display and NGS. Summer Online Seminar 2021 for Young Researchers of the Society for Biotechnology, Japan, July 2021.

8. Ami Yagimoto, Hiroya Oka, Takaaki Kojima, Hideo Nakano: Improving the Efficiency of a Comprehensive Transcriptional Regulatory Mechanism Analysis System Using a Cell-Free Protein Synthesis System. Summer Online Seminar 2021 for Young Researchers of the Society for Biotechnology, Japan, July 2021.

9. Itsuki Kawakita, Shota Kaneko, Takaaki Kojima, Toru Suzuki, Hideo Nakano: Screening for Antibodies That Bind to Gut Bacteria. 190th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, September 2021.

10. Hiroya Oka, Takaaki Kojima, Ryuji Kato, Kunio Ihara, Hideo Nakano: Construction of a Predictive Model of the Transcriptional Binding Mechanism of the Aspergillus oryzae Transcription Factor AoXlnR Based on DNA Structural Parameters around Binding Regions. 73rd Annual Meeting of the Society for Biotechnology, Japan, online, October 2021.

11. Yuka Mizutani, Hiroya Oka, Takaaki Kojima, Hideo Nakano: Comprehensive Analysis of Genes Regulated by the Kojic Acid Metabolism-Related Transcription Factor KojR in Aspergillus oryzae. 73rd Annual Meeting of the Society for Biotechnology, Japan, online, October 2021.

12. Ayumu Okazawa, Monami Kihara, Yuetsu Tanaka, Takuya Fukushima, Takaaki Kojima, Hideo Nakano: Screening for Anti-HTLV-1 Human Monoclonal Antibodies from Blood of HTLV-1-Infected Individuals. 73rd Annual Meeting of the Society for Biotechnology, Japan, online, October 2021.

13. Rio Okuda, Rena Ito, Takaaki Kojima, Hideo Nakano: Development of Antibody Affinity-Maturation Technology Using Ribosome Display with a Reconstituted Escherichia coli Cell-Free Protein Synthesis System. 73rd Annual Meeting of the Society for Biotechnology, Japan, online, October 2021.

14. Nana Odake, Jasmina Damnjanovic, Takaaki Kojima, Naoto Nemoto, Kiyotaka Hitomi, Hideo Nakano: Development of a cDNA Display Platform for Rapid Screening of Transglutaminase Substrate Specificity. 73rd Annual Meeting of the Society for Biotechnology, Japan, online, October 2021.

15. Monami Kihara, Takaaki Kojima, Tetsunari Hase, Hiroshi Ueda, Hideo Nakano: Isolation of Anti-SARS-CoV-2 Spike Human Monoclonal Antibodies from Single B Cells Derived from COVID-19 Patients Using a Cell-Free Protein Synthesis System. 2022 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, online, March 2022.

16. Hideo Yamamoto, Hideo Nakano, Takaaki Kojima, Hiroshi Ueda: Bioinformatics for Antibody Engineering. 2022 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, online, March 2022.

17. Ryo Nomura, Sho Tsuzuki, Kimitoshi Kimura, Takaaki Kojima, Motoyuki Shimizu, Masashi Kato: Prebiotic Effects of Aspergillus oryzae Contained in Fermented Foods. 2022 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, online, March 2022.

18. Hiroya Oka, Takaaki Kojima, Ryuji Kato, Kunio Ihara, Hideo Nakano: Elucidation of the Relationship between Gene-Expression Regulation by the Aspergillus oryzae Transcription Factor AoXlnR and DNA Structure near Its Binding Sites. 2022 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, online, March 2022.

19. Nanako Ogasawara, Yuka Mizutani, Hiroya Oka, Takaaki Kojima, Hideo Nakano: Comprehensive Analysis of Regulatory Mechanisms of Transcription Factors Related to Carbon-Source Metabolism in Aspergillus oryzae. 2022 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, online, March 2022.

20. Jasmina Damnjanovic, Moeri Nezu, Beixi Jia, Nana Odake, Kiyotaka Hitomi, Takaaki Kojima, Naoto Nemoto, Hideo Nakano: Comprehensive Analysis of Transglutaminase Substrate Preference by cDNA Display. 2022 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, online, March 2022.

21. Yugo Iwasaki: Enzymatic Synthesis of Plasmalogens Using an Ether-Phospholipid Desaturase. 1st Amano Enzyme Research Grant Report Meeting, Nagoya, March 2022.

22. Nana Odake, Jasmina Damnjanovic, Takaaki Kojima, Naoto Nemoto, Kiyotaka Hitomi, Maurizio Camagna, Hideo Nakano: Development of a cDNA Display Platform for Rapid Screening of Transglutaminase Substrate Preference. 1st Amano Enzyme Research Grant Report Meeting, Nagoya, March 2022.

23. T.I.K. Munaweera, Jasmina Damnjanovic, Moeri Nezu, Maurizio Camagna, Takaaki Kojima, Kiyotaka Hitomi, Hideo Nakano: Comprehensive Analysis of Transglutaminase 1 Substrate Preferences Using cDNA Display. 1st Amano Enzyme Research Grant Report Meeting, Nagoya, March 2022.

24. Hiroya Oka, Takaaki Kojima, Ryuji Kato, Kunio Ihara, Hideo Nakano: Analysis of the Relationship between Sequence-Context Information near Binding Sites of the Aspergillus oryzae Transcription Factor AoXlnR and Gene-Expression Levels. 1st Amano Enzyme Research Grant Report Meeting, Nagoya, March 2022.

25. Kyoka Ishihara, Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Development of a Phosphatidic Acid Phosphatase. 1st Amano Enzyme Research Grant Report Meeting, Nagoya, March 2022.

26. Yuma Nishikawa, Yuki Furukawa, Takaaki Kojima, Teruyo Ojima-Kato, Hideo Nakano: The MSKIK Sequence Suppresses Ribosome Stalling of Nascent Peptide Chains and Increases Protein Synthesis. 1st Amano Enzyme Research Grant Report Meeting, Nagoya, March 2022.

2020

1. Hiroya Oka: A Multifaceted Approach to Transcription-Factor Binding Mechanisms Using Various Transcription-Factor Analysis Methods and Sequence-Context Information. 1st Online Seminar for Young Researchers of the Society for Biotechnology, Japan, July 2020.

2. Phornnaphat Chertchinnapa, Yusuke Shinkai, Daorung Sila-On, Takaaki Kojima, Hideo Nakano: Rabbit Monoclonal Antibody against Epidermal Growth Factor Receptor Acquisition: Rapid Screening by Ecobody Technology. 2nd Online Seminar for Young Researchers of the Society for Biotechnology, Japan, August 2020.

3. Teruyo Ojima-Kato, Hideo Nakano, Takaaki Kojima, Satomi Nagai: Development and Practical Application of Rapid Antibody-Screening Technology Using a Cell-Free Protein Synthesis System. Biotechnology Web Symposium 2020, September 2020.

4. Takaaki Kojima: Genome-Wide Analysis of Transcriptional Regulatory Mechanisms Using Bioinformatics. GTR Retreat 2020, September 2020.

5. Hiroya Oka: Construction of a Machine-Learning Model for Predicting Transcriptional Regulatory Mechanisms in Aspergillus oryzae Based on Gene Sequence Context. GTR Retreat 2020, September 2020.

6. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Construction of a Machine-Learning Model for Predicting Genes Regulated by Transcription Factors Based on Sequence-Context Information. 187th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, September 2020.

7. Haruka Iwamoto, Tomoaki Goto, Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Analysis of the Reaction Mechanism of Microbial Phospholipase D. 187th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, September 2020.

8. Daffa Sean Adinegoro, Takaaki Kojima, Hideo Nakano: Rabbit Monoclonal Antibody Screening Targeting Membrane Proteins Using Ecobody Technology. 187th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, September 2020.

9. Phornnaphat Chertchinnapa, Yusuke Shinkai, Daorung Sila-On, Takaaki Kojima, Hideo Nakano: Rabbit Monoclonal Antibody against Epidermal Growth Factor Receptor Acquisition: Rapid Screening by Ecobody Technology. 187th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, September 2020.

10. Beixi Jia, Yoshino Uchida, Hideo Nakano, Takaaki Kojima: Isolation of Human Gut-Bacterium-Binding Antibodies and Antibody Epitope Analysis Using Ribosome Display. 187th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, September 2020.

11. Shota Kaneko, Takaaki Kojima, Hideo Nakano: Screening for Gut-Bacterium-Binding Antibodies in Rabbits. 187th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, September 2020.

12. Yuki Furukawa, Teruyo Ojima-Kato, Hideo Nakano: Study of the N-Terminal SKIK Peptide-Tag Sequence That Enhances Protein Production. 187th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, September 2020.

13. Hideo Nakano: Development and Social Implementation of High-Throughput Monoclonal-Antibody Screening Technology Using a Cell-Free Protein Synthesis System. 72nd Annual Meeting of the Vitamin Society of Japan, September 2020.

14. Beixi Jia, Yoshino Uchida, Hideo Nakano, Takaaki Kojima: Isolation of Human Gut-Bacterium-Binding Antibodies and Antibody Epitope Analysis Using Ribosome Display. 3rd Online Seminar for Young Researchers of the Society for Biotechnology, Japan, November 2020.

15. Daffa Sean Adinegoro, Takaaki Kojima, Hideo Nakano: Rabbit Monoclonal Antibody Screening Targeting Membrane Proteins Using Ecobody Technology. 3rd Online Seminar for Young Researchers of the Society for Biotechnology, Japan, November 2020.

16. Jasmina Damnjanovic, Nana Odake, Takaaki Kojima, Naoto Nemoto, Hideo Nakano: Novel cDNA Display Platform for Activity-Based Selection in Protein Engineering. 2021 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sendai, March 2021.

17. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Comprehensive Analysis of the Regulatory Mechanism of the Aspergillus oryzae Transcription Factor AoXlnR Using DNA Structural Parameters. 2021 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sendai, March 2021.

18. Koki Yasufuku, Takaaki Kojima, Yoshiaki Inukai, Hiroya Oka, Hideo Nakano: Analysis of the Regulatory Mechanism of Rice Lateral-Root Meristem Size Using Comprehensive Transcriptional Regulatory Network Analysis Technology. 2021 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sendai, March 2021.

19. Monami Kihara, Daffa Sean Adinegoro, Takaaki Kojima, Tetsunari Hase, Atsushi Enomoto, Hideo Nakano: Development of a Method for Obtaining Rabbit Monoclonal Antibodies Using DNA Immunization. 2021 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sendai, March 2021.

20. Haruka Iwamoto, Tomoaki Goto, Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Analysis of the Reaction Mechanism of Microbial Phospholipase D. 2021 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sendai, March 2021.

21. Haruna Sakagami, Tomokazu Ito, Yugo Iwasaki, Hisashi Hemmi, Toru Yoshimura: Structural and Functional Studies of D-Amino Acid N-Acetyltransferase (HPA3) from Saccharomyces cerevisiae. 2021 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sendai, March 2021.

22. Haruka Minagawa, Takuya Katayama, Hiroya Oka, Masahiro Ogawa, Takaaki Kojima, Hideo Nakano, Yasuhito Koyama, Katsuhiko Kitamoto, Jun-ichi Maruyama: Analysis of the Regulatory Mechanism of Sclerotium Formation by the Transcription Factors TrsA and TrsB in Aspergillus oryzae. 2021 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sendai, March 2021.

2019

1. Takaaki Kojima: Development of Technologies for Screening Functional Biomacromolecules Using Ultra-Miniaturized Biochemical Reaction Systems and Bioinformatics. 185th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Shizuoka, June 2019.

2. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Comprehensive Analysis of the Relationship between Binding Sequences and Transcriptional Regulation by the Filamentous-Fungal Transcription Factor AoXlnR. Summer Seminar 2019 for Young Researchers of the Society for Biotechnology, Japan, Takashima, July 2019.

3. Rena Ito, Almasul Alfi, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: High-Efficiency Production of an Antibody-Enzyme Fusion Protein (Zipbodyzyme) Using the PURE System. Summer Seminar 2019 for Young Researchers of the Society for Biotechnology, Japan, Takashima, July 2019.

4. Koki Yasufuku, Takaaki Kojima, Yoshiaki Inukai, Hideo Nakano: Comprehensive Analysis of the Regulatory Mechanism of Lateral-Root Meristem Size by the Rice Transcription Factor OsbZIP1. Summer Seminar 2019 for Young Researchers of the Society for Biotechnology, Japan, Takashima, July 2019.

5. Hideo Nakano, Teruyo Ojima-Kato, Masashi Ohuchi, Takaaki Kojima: Social Implementation of a Rapid Monoclonal-Antibody Discovery Platform Technology. 71st Annual Meeting of the Society for Biotechnology, Japan, Okayama, September 2019.

6. Takaaki Kojima, Motoyuki Shimizu, Yasunori Baba, Hideo Nakano, Masashi Kato: Improvement of the Host Gut Microbiota and Alleviation of Colitis through Consumption of Aspergillus oryzae. 71st Annual Meeting of the Society for Biotechnology, Japan, Okayama, September 2019.

7. Rena Ito, Almasul Alfi, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Efficient Synthesis of an Antibody-Enzyme Fusion Protein (Zipbodyzyme) Using a Reconstituted Cell-Free Protein Synthesis System. 71st Annual Meeting of the Society for Biotechnology, Japan, Okayama, September 2019.

8. Yuki Furukawa, Teruyo Ojima-Kato, Hideo Nakano: Study of the N-Terminal SKIK Peptide-Tag Sequence That Enhances Protein Production. 71st Annual Meeting of the Society for Biotechnology, Japan, Okayama, September 2019.

9. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Analysis of the Relationship between Binding-Sequence Context and Expression Response of a Filamentous-Fungal Transcription Factor Using Data Mining. 71st Annual Meeting of the Society for Biotechnology, Japan, Okayama, September 2019.

10. Koki Yasufuku, Takaaki Kojima, Yoshiaki Inukai, Hideo Nakano: Elucidation of the Regulatory Mechanism of Rice Lateral-Root Meristem Size Using Comprehensive Transcriptional Regulatory Network Analysis Technology. 71st Annual Meeting of the Society for Biotechnology, Japan, Okayama, September 2019.

11. Yugo Iwasaki, Hideo Nakano, Jasmina Damnjanovic: Acyl Chain That Matters: Introducing sn-2 Acyl Chain Preference to a Phospholipase D by Protein Engineering. 15th ISBAB, Hiroshima, September 2019.

12. Daorung Sila-On, Yusuke Shinkai, Takaaki Kojima, Hideo Nakano: The Novel Rabbit Monoclonal Antibody (mAb) against Swine Influenza Virus (SIV) Using Ecobody Technology. 2019 Joint Kobe Meeting of the Kansai and Chubu Branches, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kobe, September 2019.

13. Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University; Laboratory of Applied Microbiology, Graduate School of Agriculture, Meijo University; Laboratory of Environmental Biotechnology, Research Institute for Bioresources, Ishikawa Prefectural University: Food Functions of Brewing Microorganisms and Aspergillus oryzae: Improvement of Gut Microbiota and Alleviation of Colitis. 9th Workshop of the Center for Advanced Medicine and Clinical Research, Nagoya University, Nagoya, January 2020.

14. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Analysis of the Binding Mechanism of an Aspergillus oryzae Transcription Factor Using DNA Structural Information. 2020 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Fukuoka, March 2020. [Cancelled]

15. Bo Zhu, Rui Gan, Maria D. Cabezas, Takaaki Kojima, Robert Nicol, Michael C. Jewett, Hideo Nakano: CroP-LET: A Rapid Linear Expression Template Protection by DNA Binding Protein scCro Enhances Yields of Cell-Free Protein Synthesis Systems. 2020 Sakura-Bio Online Meeting, Nagoya, March 2020.

16. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Integrative Mining for Comprehensive Analysis of the Gene Expression System Regulated by a Transcription Factor, AoXlnR in Aspergillus oryzae. 2020 Sakura-Bio Online Meeting, Nagoya, March 2020.

17. Daorung Sila-On, Phornnaphat Chertchinnapa, Yusuke Shinkai, Takaaki Kojima, Hideo Nakano: Development of Sandwich-ELISA Using Rabbit Monoclonal Antibody (mAb) by Ecobody Technology. 2020 Sakura-Bio Online Meeting, Nagoya, March 2020.

18. Phornnaphat Chertchinnapa, Yusuke Shinkai, Daorung Sila-On, Takaaki Kojima, Hideo Nakano: Rabbit Monoclonal Antibody against Epidermal Growth Factor Receptor Acquisition: Rapid Screening by Ecobody Technology. 2020 Sakura-Bio Online Meeting, Nagoya, March 2020.

2018

1. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Comprehensive Analysis of Genes Regulated by the Aspergillus oryzae Transcription Factor AoXlnR Using Data Mining. Summer Seminar 2018 for Young Researchers of the Society for Biotechnology, Japan, Kitami, July 2018.

2. Teruyo Ojima-Kato, Hiroto Tamura, Hideo Nakano: Enhancement of Monoclonal-Antibody Production by an N-Terminal SKIK Peptide and Elucidation of Its Mechanism. 70th Annual Meeting of the Society for Biotechnology, Japan, Osaka, September 2018.

3. Yusuke Shinkai, Shiomi Morishita, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Development of a Rapid Method for Obtaining Rabbit Monoclonal Antibodies Using the Ecobody Method. 70th Annual Meeting of the Society for Biotechnology, Japan, Osaka, September 2018.

4. Yuka Komori, Yoshino Uchida, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Rapid Isolation of Human Monoclonal Antibodies by the Ecobody Method and Their Production in Escherichia coli. 70th Annual Meeting of the Society for Biotechnology, Japan, Osaka, September 2018.

5. Yohei Fujii, Hiroya Oka, Takuya Katayama, Takaaki Kojima, Hideo Nakano, Jun-ichi Maruyama: Identification of DNA-Binding Sequences of Transcription Factors Involved in Filamentous-Fungal Differentiation Using the gSELEX Method. 70th Annual Meeting of the Society for Biotechnology, Japan, Osaka, September 2018.

6. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Comprehensive Analysis of the Gene-Expression Regulatory System Controlled by the Aspergillus oryzae Transcription Factor XlnR. 70th Annual Meeting of the Society for Biotechnology, Japan, Osaka, September 2018.

7. Takaaki Kojima, Hideo Nakano: Aspiring to Become a Bioinformatician: Analysis of Transcriptional Regulatory Mechanisms Using High-Speed DNA Sequencing. 7th CHUBU Discussion Meeting, Chubu Branch of the Society for Biotechnology, Japan, Motosu, September 2018.

8. Hideo Nakano, Teruyo Ojima-Kato, Takaaki Kojima, Panwad Ritthisan: Development of Fab Antibody-Enzyme Fusion Technology Using a Protein-Expression Enhancing Tag and a Protein-Protein Interaction Enhancing Tag. 50th Autumn Meeting of the Society of Chemical Engineers, Japan, Kagoshima, September 2018.

9. Yugo Iwasaki, Jasmina Damnjanovic: Enzymatic Preparation of “Difficult-to-Synthesize” Bioactive Phospholipids. JOCS-AOCS Joint Symposium 2018, Kobe, September 2018.

10. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Comprehensive Analysis of the Gene-Expression Regulatory Mechanism Controlled by the Aspergillus oryzae Transcription Factor XlnR Using Data Mining. 2018 Chubu Branch Meeting of the Society for Biotechnology, Japan, Nagoya, November 2018.

11. Hiroya Oka: Comprehensive Investigation of the Gene Expression System Regulated by Transcription Factor AoXlnR in Aspergillus oryzae. International Collaborative Program in Agricultural Sciences between The University of Western Australia and Nagoya University, Joint PhD Program Signing Ceremony & Commemoration Workshop, Nagoya, December 2018.

12. Takaaki Kojima: Development of Technologies for Screening Functional Biomacromolecules Using Ultra-Miniaturized Biochemical Reaction Systems and Bioinformatics. 2019 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Tokyo, March 2019.

13. Almasul Alfi, Shunta Ogawa, Takaaki Kojima, Teruyo Ojima-Kato, Jasmina Damnjanovic, Hideo Nakano: The Production and Refolding of Fab Antibody Fused with NanoLuc Expressed in Escherichia coli. 2019 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Tokyo, March 2019.

14. Yuhei Ishihara, Hiroya Oka, Jun-ichi Maruyama, Takaaki Kojima, Hideo Nakano: Genome-Wide Analysis of Binding Sites of Filamentous-Fungal Transcription Factors Using a Dimerization Tag. 2019 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Tokyo, March 2019.

15. Sho Tsuzuki, Kazuhiro Yamada, Mai Sakakibara, Mao Nagasawa, Toshiya Hayashi, Takaaki Kojima, Hideo Nakano, Motoyuki Shimizu, Masashi Kato: Changes in Host Gut Microbiota and Alleviation of Colitis by Consumption of Aspergillus oryzae. 2019 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Tokyo, March 2019.

16. Teruyo Ojima-Kato: Ecobody Technology: A Rapid and Robust Monoclonal Antibody Screening Method from Single B Cells Using Cell-Free Protein Synthesis. 2019 Sakura-Bio Meeting, Nagoya, March 2019.

17. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Integrative Mining for Comprehensive Analysis of the Gene Expression System Regulated by a Transcription Factor, AoXlnR in Aspergillus oryzae. 2019 Sakura-Bio Meeting, Nagoya, March 2019.

18. Almasul Alfi, Shunta Ogawa, Takaaki Kojima, Teruyo Ojima-Kato, Jasmina Damnjanovic, Hideo Nakano: The Production and Refolding of Fab Antibody Fused with NanoLuc Expressed in Escherichia coli. 2019 Sakura-Bio Meeting, Nagoya, March 2019.

2017

1. Takaaki Kojima, Hideo Nakano: Evaluation and Application of Precise Spatial Arrangement of Proteins Using a DNA-Binding Tag by BLI. ForteBio User Meeting 2017, Tokyo, May 2017.

2. Yugo Iwasaki, Jasmina Damnjanovic, Michiko Muraki, Hideo Nakano: Improving the Positional Specificity and the Reaction Efficiency of Phospholipase D-Mediated Phosphatidylinositol Synthesis. 2017 AOCS Annual Meeting, Orlando, USA, May 2017.

3. Teruyo Ojima-Kato: Studies on Microbial Production of Useful Proteins and Their Industrial Applications. 179th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Minamiminowa, June 2017.

4. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Genome-Wide Analysis of XlnR-Binding Sites in Aspergillus oryzae and Comprehensive Identification of Its Regulated Genes. Summer Seminar 2017 for Young Researchers of the Society for Biotechnology, Japan, Fukuyama, July 2017.

5. Teruyo Ojima-Kato: Study of Enhanced Protein Expression Using an N-Terminal SKIK Peptide Tag. 2017 Chubu Branch Meeting of the Society for Biotechnology, Japan, Nagoya, September 2017.

6. Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Acyl Chain That Matters: Turning a Phospholipase D into a Lysophospholipase D by Protein Engineering. 69th Annual Meeting of the Society for Biotechnology, Japan, Tokyo, September 2017.

7. Nozomi Matsunaga, Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Enzymatic Synthesis of Phosphatidylthreonine Using Engineered Phospholipase D. 69th Annual Meeting of the Society for Biotechnology, Japan, Tokyo, September 2017.

8. Yoshino Uchida, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Isolation of Anti-Influenza Monoclonal Antibodies from Single Human B Cells Using the Ecobody Method. 69th Annual Meeting of the Society for Biotechnology, Japan, Tokyo, September 2017.

9. Sho Tsuzuki, Kazuhiro Yamada, Mayuka Takada, Shunsuke Murata, Moeko Marui, Mao Nagasawa, Toshiya Hayashi, Takuya Katayama, Jun-ichi Maruyama, Takaaki Kojima, Hideo Nakano, Motoyuki Shimizu, Masashi Kato: Effects of Consuming Live Aspergillus oryzae on the Host Gut Microbiota and Intestinal Metabolites. 69th Annual Meeting of the Society for Biotechnology, Japan, Tokyo, September 2017.

10. Shiomi Morishita, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Development of a Rapid Method for Obtaining Rabbit Monoclonal Antibodies from Single B Cells. 49th Autumn Meeting of the Society of Chemical Engineers, Japan, Nagoya, September 2017.

11. Teruyo Ojima-Kato, Takaaki Kojima, Hiroto Tamura, Hideo Nakano: Rapid Isolation of Monoclonal Antibodies from Single B Cells and Their Production in Escherichia coli. 49th Autumn Meeting of the Society of Chemical Engineers, Japan, Nagoya, September 2017.

12. Hideo Nakano, Teruyo Ojima-Kato, Takaaki Kojima: Ecobody Method: Rapid and Low-Cost Technology for Obtaining Monoclonal Antibodies from Single B Cells. 49th Autumn Meeting of the Society of Chemical Engineers, Japan, Nagoya, September 2017.

13. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Comprehensive Identification of Genes Regulated by the Aspergillus oryzae Transcription Factor XlnR. 180th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2017.

14. Hideo Nakano: Ecobody Technology: A Novel Antibody Screening and Production Method of Monoclonal Antibodies by Single B-Cell RT-PCR and Escherichia coli In Vivo and In Vitro Expression Systems. 2017 KSBB Fall Meeting and International Symposium, Busan, Korea, October 2017.

15. Hideo Nakano, Teruyo Kato, Takaaki Kojima: Ecobody Technology: A Novel Antibody Screening and Production Method of Monoclonal Antibodies by Single B-Cell RT-PCR and Escherichia coli In Vivo and In Vitro Expression Systems. Italy-Japan Joint Symposium: New Trends in Enzyme and Microbial Science in the Translational Biology Era, Napoli, Italy, October 2017.

16. Jasmina Damnjanovic, Nozomi Matsunaga, Michiko Muraki, Arisa Inoue, Hideo Nakano, Yugo Iwasaki: Protein Engineering of Phospholipase D for Enzymatic Synthesis of Bioactive Phospholipids. 2nd Research Meeting of the Genome-Epigenome Research Project, Osaka, December 2017.

17. Hideo Nakano, Teruyo Ojima-Kato, Shiomi Morishita, Yoshino Uchida, Panwad Ritthisan, Takaaki Kojima: Ecobody Technology: A Novel Rapid Method for Obtaining Monoclonal Antibodies from Single B Cells Using a Cell-Free Protein Synthesis System and Escherichia coli Expression. 2017 Joint Annual Meeting of Life Science Societies, 40th Annual Meeting of the Molecular Biology Society of Japan and 90th Annual Meeting of the Japanese Biochemical Society, Kobe, December 2017.

18. Panwad Ritthisan, Teruyo Ojima-Kato, Jasmina Damnjanovic, Takaaki Kojima, Hideo Nakano: Soluble Expression and Characterization of Fragment Antigen-Binding (Fab) Derivative in Escherichia coli Cytoplasm. Antibody Engineering & Therapeutics, San Diego, December 2017.

19. Miho Sato, Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Development of a Method for Synthesizing Ether-Type Phospholipids. 8th Interdisciplinary Lipid Creation Research Meeting, Higashihiroshima, January 2018.

20. Tomoaki Goto, Natsuki Hattori, Hideo Nakano, Yugo Iwasaki: Development of a Method for Analyzing the Stereospecific Positional Distribution of Fatty Acids Bound to Lipids. 8th Interdisciplinary Lipid Creation Research Meeting, Higashihiroshima, January 2018.

21. Hiroya Oka, Takaaki Kojima, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Comprehensive Analysis and Data Mining of Genes Regulated by the Metabolic Transcription Factor XlnR from Aspergillus oryzae. 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, March 2018.

22. Yusuke Shinkai, Shiomi Morishita, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Development of a Method for Obtaining Rabbit Monoclonal Antibodies Using the Ecobody Method. 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, March 2018.

23. Yuka Komori, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Isolation of Anti-Influenza Monoclonal Antibodies from Single Human B Cells by the Ecobody Method and Their High-Level Expression in Escherichia coli. 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, March 2018.

24. Panwad Ritthisan, Teruyo Ojima-Kato, Jasmina Damnjanovic, Takaaki Kojima, Hideo Nakano: The Production and Characterization of Fab Antibody Fused with Escherichia coli Alkaline Phosphatase Expressed in Escherichia coli Oxidative Cytoplasm. 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, March 2018.

25. Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Engineered Phospholipase D with High Specificity for Lysophospholipids and Diacylphospholipids with Short-Chain sn-2 Groups. 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, March 2018.

26. Takaaki Kojima, Hideo Nakano: Precise Spatial Arrangement of Target Proteins Using a DNA-Binding Tag for Screening Novel Functional Molecules. 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, March 2018.

27. Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Rapid Isolation of Monoclonal Antibodies from Single B Cells and Their Production in Escherichia coli. 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, March 2018.

2016

1. Hideo Nakano: Zipbody: Fab Antibodies Using a Leucine Zipper and Their Applications. 5th Neo-Biomolecular Research Meeting, Kagoshima, June 2016.

2. Kenta Hayashi, Eri Ota, Bo Zhu, Takaaki Kojima, Hideo Nakano: Activity Analysis of D-Amino Acid Oxidase Using an Enzyme-Alignment Technology Based on DNA-Binding Protein Tags. Summer Seminar 2016 for Young Researchers of the Society for Biotechnology, Japan, Fuchu, July 2016.

3. Natsuki Hattori, Hideo Nakano, Yugo Iwasaki: Construction of an Escherichia coli Expression System for Candida antarctica Lipase A. Summer Seminar 2016 for Young Researchers of the Society for Biotechnology, Japan, Fuchu, July 2016.

4. Miho Sato, Arisa Inoue, Masanori Adachi, Hideo Nakano, Jasmina Damnjanovic, Yugo Iwasaki: Enzymatic Synthesis of Functional Glycophospholipids. Summer Seminar 2016 for Young Researchers of the Society for Biotechnology, Japan, Fuchu, July 2016.

5. Shiomi Morishita, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Isolation of Rabbit Monoclonal Antibodies against Shiga Toxin 2. Summer Seminar 2016 for Young Researchers of the Society for Biotechnology, Japan, Fuchu, July 2016.

6. Yoshino Uchida, Teruyo Ojima-Kato, Hideo Nakano: Efficient Antibody Synthesis Using a Cell-Free Protein Synthesis System. Summer Seminar 2016 for Young Researchers of the Society for Biotechnology, Japan, Fuchu, July 2016.

7. Ayako Nakane, Bo Zhu, Hideo Nakano, Takaaki Kojima: Effects of Disulfide Bonds on Protein Folding Using Horseradish Peroxidase as a Model. Summer Seminar 2016 for Young Researchers of the Society for Biotechnology, Japan, Fuchu, July 2016.

8. Panwad Ritthisan, Teruyo Ojima-Kato, Satomi Nagai, Takaaki Kojima, Hideo Nakano: Expression, Solubilization and Refolding of Antigen-Binding Fragments of Antibodies Fused with Leucine Zipper in Escherichia coli. 6th International Conference on Biological, Chemical & Environmental Sciences, Pattaya, Thailand, August 2016.

9. J. Damnjanovic, A. Inoue, M. Adachi, H. Nakano, Y. Iwasaki: One-Step Enzymatic Synthesis of 1-Phosphatidyl-β-D-glucose and Its Purification. 8th International Congress on Biocatalysis, Hamburg, Germany, August 2016.

10. A. Alfi, B. Zhu, J. Damnjanovic, T. Kojima, Y. Iwasaki, H. Nakano: Chaperone Co-Expression and In Vitro Maturation to Obtain Active Manganese Peroxidase Using an E. coli Host. 8th International Congress on Biocatalysis, Hamburg, Germany, August 2016.

11. Panwad Ritthisan, Teruyo Ojima-Kato, Satomi Nagai, Takaaki Kojima, Hideo Nakano: Refolding of Antigen-Binding Fragments with the Leucine Zipper Domain from Escherichia coli Inclusion Bodies. 177th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, September 2016.

12. Natsuki Hattori, Hideo Nakano, Yugo Iwasaki: Construction of an Escherichia coli Expression System for Candida antarctica Lipase A. 177th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, September 2016.

13. Shiomi Morishita, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Development of a Rapid Method for Obtaining Rabbit Monoclonal Antibodies from Single B Cells. 177th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, September 2016.

14. Yoshino Uchida, Teruyo Ojima-Kato, Hideo Nakano: Study of a Novel Method for Obtaining Human Monoclonal Antibodies. 177th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, September 2016.

15. Takaaki Kojima, Hideo Nakano: High-Throughput Enzyme Evolution Technology Using Miniaturized Biochemical Reaction Cascades. 89th Annual Meeting of the Japanese Biochemical Society, Sendai, September 2016.

16. Takaaki Kojima, Hideo Nakano: Analysis of Transcriptional Regulatory Networks Using High-Speed DNA Sequencing and Bioinformatics. 68th Annual Meeting of the Society for Biotechnology, Japan, Toyama, September 2016.

17. Natsuki Hattori, Hideo Nakano, Yugo Iwasaki: Extracellular Production of Candida antarctica Lipase A by Recombinant Escherichia coli. 68th Annual Meeting of the Society for Biotechnology, Japan, Toyama, September 2016.

18. Teruyo Ojima-Kato, Hideo Nakano: Application and Mechanistic Analysis of an N-Terminal Peptide Tag for Increasing the Expression of Difficult-to-Express Proteins. 68th Annual Meeting of the Society for Biotechnology, Japan, Toyama, September 2016.

19. Hideo Nakano, Akihiro Mori, Teruyo Ojima-Kato, Takaaki Kojima: Development of a Novel Antibody-Enzyme Fusion Protein, Zipbodyzyme. 68th Annual Meeting of the Society for Biotechnology, Japan, Toyama, September 2016.

20. Shiomi Morishita, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Isolation of Rabbit Monoclonal Antibodies against Shiga Toxin 2. 68th Annual Meeting of the Society for Biotechnology, Japan, Toyama, September 2016.

21. Panwad Ritthisan, Teruyo Ojima-Kato, Satomi Nagai, Takaaki Kojima, Hideo Nakano: Expression and Refolding of Antigen-Binding Fragments (Fab) with the Leucine Zipper in E. coli. 68th Annual Meeting of the Society for Biotechnology, Japan, Toyama, September 2016.

22. Yugo Iwasaki: Engineering of Streptomyces Phospholipase D and Its Application to Phospholipid Synthesis. 2nd Research Meeting on the Development and Application of Novel Fatty-Acid Analysis Methods for Extending Healthy Lifespan, Nagoya, October 2016.

23. Michiko Muraki, Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Improvement of Enzymatic Phosphatidylinositol Synthesis by Addition of High Concentrations of Salt. 47th Joint Autumn Meeting of Chemical Societies in the Chubu Area, Toyohashi, November 2016.

24. Yugo Iwasaki: Enzymatic Synthesis of Functional Phospholipids. 5th CHUBU Discussion Meeting, Chubu Branch of the Society for Biotechnology, Japan, Suzuka, December 2016.

25. Hideo Nakano, Jumpei Hata, Kenta Hayashi, Takaaki Kojima: Precise Spatial Arrangement of Proteins Using DNA-Binding Tags and Its Applications. 82nd Annual Meeting of the Society of Chemical Engineers, Japan, Tokyo, March 2017.

26. Shiomi Morishita, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Development of a Rapid Method for Obtaining Rabbit Monoclonal Antibodies from Single B Cells. 82nd Annual Meeting of the Society of Chemical Engineers, Japan, Tokyo, March 2017.

27. Yoshino Uchida, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Isolation of Human Monoclonal Antibodies against Lactic Acid Bacteria from Single Human B Cells Using an Escherichia coli Expression System. 2017 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2017.

28. Hiroya Oka, Takaaki Kojima, Tomoya Kajikawa, Hiroki Goto, Teruyuki Niimi, Kunio Ihara, Hideo Nakano: Genome-Wide Analysis of Binding Sites of the Rhinoceros Beetle Transcription Factor Dsx Using High-Speed DNA Sequencing. 2017 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2017.

29. JiCheng Fan, Jasmina Damnjanovic, Takaaki Kojima, Naoto Nemoto, Hideo Nakano: cDNA Display Using an E. coli-Based Translational System. 2017 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2017.

30. Kazuhiro Yamada, Sho Tsuzuki, Mayuka Takada, Shunsuke Murata, Moeko Marui, Mao Nagasawa, Toshiya Hayashi, Takuya Katayama, Jun-ichi Maruyama, Takaaki Kojima, Hideo Nakano, Motoyuki Shimizu, Masashi Kato: Effects of Consuming Live Aspergillus oryzae on the Intestinal Environment. 2017 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2017.

2015

1. Hideo Nakano, Takaaki Kojima: Development of Cell-Free Nanostructured Biological Functional Systems: Application to Screening of Artificial Recognition Molecules. Artificial Recognition Molecule Research Meeting, Osaka, June 2015.

2. Marsel Murzabaev, Takaaki Kojima, Isao Kobayashi, Hideo Nakano: Screening of Promoter Libraries Using Microbead Display. Summer Seminar 2015 for Young Researchers of the Society for Biotechnology, Japan, Kitanagoya, July 2015.

3. Michiko Muraki, Hideo Nakano, Yugo Iwasaki: Improvement of Enzymatic Phosphatidylinositol Synthesis. Summer Seminar 2015 for Young Researchers of the Society for Biotechnology, Japan, Kitanagoya, July 2015.

4. Ayana Ujiie, Hideo Nakano, Yugo Iwasaki: Extracellular Secretory Expression of Candida antarctica Lipase B Using Recombinant Escherichia coli. Summer Seminar 2015 for Young Researchers of the Society for Biotechnology, Japan, Kitanagoya, July 2015.

5. Takato Ota, Yugo Iwasaki: Development of a Phosphatidic Acid Phosphatase. Summer Seminar 2015 for Young Researchers of the Society for Biotechnology, Japan, Kitanagoya, July 2015.

6. Arisa Inoue, Masanori Adachi, Hideo Nakano, Jasmina Damnjanovic, Yugo Iwasaki: Enzymatic Synthesis of 1-Phosphatidyl-β-D-glucose. Summer Seminar 2015 for Young Researchers of the Society for Biotechnology, Japan, Kitanagoya, July 2015.

7. Jumpei Hata, Takuro Mizoguchi, Takaaki Kojima, Kiyotaka Hitomi, Hideo Nakano: Development of a Novel Assay Method for Transglutaminase. Summer Seminar 2015 for Young Researchers of the Society for Biotechnology, Japan, Kitanagoya, July 2015.

8. Teruyo Ojima-Kato, Dai Hashimura, Satomi Nagai, Takaaki Kojima, Hideo Nakano: Generation of Rabbit Monoclonal Antibodies Using Escherichia coli Live-Cell and Cell-Free Expression Systems. Summer Seminar 2015 for Young Researchers of the Society for Biotechnology, Japan, Kitanagoya, July 2015.

9. Shotaro Kubo, Kansuke Fukui, Hiroaki Yamamoto, Teruyo Ojima-Kato, Hideo Nakano: Improvement of Antibody Expression Using a Cell-Free Protein Synthesis System. Summer Seminar 2015 for Young Researchers of the Society for Biotechnology, Japan, Kitanagoya, July 2015.

10. Keisuke Honma, Teruyo Ojima-Kato, Takaaki Kojima, Hiroshi Ueda, Hideo Nakano: Improving Soluble Expression of Antibody Fusion Proteins in Escherichia coli by Addition of Chaperones and Tag Sequences. Summer Seminar 2015 for Young Researchers of the Society for Biotechnology, Japan, Kitanagoya, July 2015.

11. Bo Zhu, Takuro Mizoguchi, Takaaki Kojima, Hideo Nakano: Development of an Ultra-High-Throughput Screening System of Peroxidase by Using Microbead Display. Summer Seminar 2015 for Young Researchers of the Society for Biotechnology, Japan, Kitanagoya, July 2015.

12. Akihiro Mori, Shoichi Hara, Tomohiro Sugawara, Takehiko Sahara, Satoru Ogiya, Yasumasa Kawarasaki, Takaaki Kojima, Hideo Nakano: Selection of a Secretion Signal for Phanerochaete chrysosporium Endoglucanase from a Yeast Signal-Peptide Library. Summer Seminar 2015 for Young Researchers of the Society for Biotechnology, Japan, Kitanagoya, July 2015.

13. Y. Iwasaki, J. Damnjanovic, H. Nakano: Phospholipase D as a Catalyst: Application in Phospholipid Synthesis. 6th International Conference on Fermentation Technology for Value-Added Agricultural Products, Khon Kaen, Thailand, July 2015.

14. T. Ojima-Kato, K. Fukui, T. Kojima, H. Nakano: Leucine Zipper-Fused Fab: Enhancement of Active Fab Formation in E. coli In Vitro and In Vivo Expression Systems. 29th Annual Symposium of the Protein Society, Barcelona, Spain, July 2015.

15. Yoshiaki Inukai, Takaaki Kojima: Functions and Networks of Lateral-Root Formation Genes Contributing to Drought Tolerance in Rice. Genome Support Expanded Group Meeting, Kyoto, August 2015.

16. Hideo Nakano: Active-Protein Synthesis and Screening Methods Combining Cell-Free Protein Synthesis Systems and Live-Cell Expression Systems. 2015 Chubu Branch Meeting of the Society for Biotechnology, Japan, Nagoya, September 2015.

17. Bo Zhu, Takaaki Kojima, Hideo Nakano: Development of an Activity-Based Ultra-High-Throughput Screening System of Peroxidase Using Microbead Display. 2015 Chubu Branch Meeting of the Society for Biotechnology, Japan, Nagoya, September 2015.

18. Marsel Murzabaev, Takaaki Kojima, Hideo Nakano: Handmade Microfluidic Device for Ultra-High-Throughput Screening of Promoter Libraries. 2015 Chubu Branch Meeting of the Society for Biotechnology, Japan, Nagoya, September 2015.

19. Hideo Nakano, Teruyo Kato, Kansuke Fukui, Takaaki Kojima: Zipbody: A Novel Fab Derivative Easily Expressed in E. coli. 47th Autumn Meeting of the Society of Chemical Engineers, Japan, Sapporo, September 2015.

20. Bo Zhu, Takuro Mizoguchi, Takaaki Kojima, Hideo Nakano: Development of an Activity-Based Ultra-High-Throughput Screening System of Peroxidase Using Microbead Display. Enzyme Engineering XXIII, St. Petersburg, USA, September 2015.

21. Almasul Alfi, Bo Zhu, Jasmina Damnjanovic, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano: Expression of Soluble Manganese Peroxidase in Chaperone-Overexpressing E. coli and In Vitro Maturation by ATP-Dependent Chaperone Release. 2015 Joint Meeting of the Chubu and Kansai Branches, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Imizu, September 2015.

22. Jasmina Damnjanovic, Chisato Kuroiwa, Ken Ishida, Hidetoshi Tanaka, Michiko Muraki, Hideo Nakano, Yugo Iwasaki: Directing Positional Specificity in Phospholipase D-Catalyzed Synthesis of 1-Phosphatidylinositol. 2015 Joint Meeting of the Chubu and Kansai Branches, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Imizu, September 2015.

23. Arisa Inoue, Masanori Adachi, Hideo Nakano, Jasmina Damnjanovic, Yugo Iwasaki: Enzymatic Synthesis of 1-Phosphatidyl-β-D-glucose. 2015 Joint Meeting of the Chubu and Kansai Branches, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Imizu, September 2015.

24. Hideo Nakano, Takaaki Kojima: Development of Cell-Free Nanostructured Biological Functional Systems. 6th General Meeting of the Research Area “Establishing a Synthetic Biology Foundation for Understanding Dynamic and Multifactorial Biomolecular Networks,” Fukuoka, October 2015.

25. Hideo Nakano, Takaaki Kojima, Teruyo Ojima-Kato: Leucine-Zipper-Fused Antibodies Readily Synthesized in Cell-Free Protein Synthesis Systems and in the Escherichia coli Cytoplasm. 67th Annual Meeting of the Society for Biotechnology, Japan, Kagoshima, October 2015.

26. Jasmina Damnjanovic, Chisato Kuroiwa, Ken Ishida, Hidetoshi Tanaka, Hideo Nakano, Yugo Iwasaki: Protein Engineering and Temperature Control as Means of Directing Positional Specificity in Phospholipase D-Catalyzed Synthesis of 1-Phosphatidylinositol. 67th Annual Meeting of the Society for Biotechnology, Japan, Kagoshima, October 2015.

27. Michiko Muraki, Hideo Nakano, Yugo Iwasaki: Improvement of Enzymatic Phosphatidylinositol Synthesis. 67th Annual Meeting of the Society for Biotechnology, Japan, Kagoshima, October 2015.

28. Teruyo Ojima-Kato, Satomi Nagai, Hideo Nakano: Enhancement of Difficult-to-Express Protein Production by Addition of an N-Terminal Peptide Tag. 67th Annual Meeting of the Society for Biotechnology, Japan, Kagoshima, October 2015.

29. Akihiro Mori, Teruyo Ojima-Kato, Shingo Fuchi, Takaaki Kojima, Hideo Nakano: Development of a Novel Immunological Detection Method Using Near-Infrared Fluorescent Glass. 67th Annual Meeting of the Society for Biotechnology, Japan, Kagoshima, October 2015.

30. Takaaki Kojima, Tetsuo Kobayashi, Hideo Nakano: Genome-Wide Screening of Binding Sites for the Filamentous-Fungal Transcription Factor AmyR Using SELEX-Seq. 67th Annual Meeting of the Society for Biotechnology, Japan, Kagoshima, October 2015.

31. Almasul Alfi, Bo Zhu, Jasmina Damnjanovic, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano: Expression of Manganese Peroxidase from Phanerochaete chrysosporium in Chaperone-Overexpressing Escherichia coli. 67th Annual Meeting of the Society for Biotechnology, Japan, Kagoshima, October 2015.

32. Jumpei Hata, Takuro Mizoguchi, Takaaki Kojima, Kiyotaka Hitomi, Hideo Nakano: Expression of Mouse TG2 in a Cell-Free Protein Synthesis System. 67th Annual Meeting of the Society for Biotechnology, Japan, Kagoshima, October 2015.

33. Shotaro Kubo, Kansuke Fukui, Hiroaki Yamamoto, Teruyo Ojima-Kato, Hideo Nakano: Improvement of Fab-LZ Expression Using a Cell-Free Protein Synthesis System. 67th Annual Meeting of the Society for Biotechnology, Japan, Kagoshima, October 2015.

34. Takaaki Kojima, Hideo Nakano: Development of High-Throughput Screening Technologies for Functional Nucleic Acids and Proteins Using the Bead Display Method. 2015 Tenure-Track Workshop, Graduate School of Science and Engineering, University of Toyama, Toyama, November 2015.

35. Arisa Inoue, Masanori Adachi, Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Enzymatic Synthesis of Phosphatidyl-β-1-glucose. 6th Interdisciplinary Lipid Creation Research Meeting, Tokushima, January 2016.

36. Michiko Muraki, Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Improvement of Enzymatic Phosphatidylinositol Synthesis by Addition of High Concentrations of Salt. 6th Interdisciplinary Lipid Creation Research Meeting, Tokushima, January 2016.

37. Akihiro Mori, Teruyo Ojima-Kato, Shingo Fuchi, Takaaki Kojima, Hideo Nakano: Development of a Novel Immunosorbent Homogeneous Assay System Using Luciferase-Antibody Complex and Dye for Luminescence Absorption. 251st American Chemical Society National Meeting & Exposition, San Diego, USA, March 2016.

38. Kenta Hayashi, Eri Ota, Bo Zhu, Takaaki Kojima, Hideo Nakano: Enzyme-Alignment Technology Using DNA-Binding Protein Tags and Its Application to Bead Display. 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sapporo, March 2016.

39. Kazuhiro Tanaka, Teruyo Ojima-Kato, Shinichi Kaiya, Shiro Miyake, Shingo Fuchi, Hideo Nakano, Hiroto Tamura: Establishment of a Foodborne-Pathogen Detection Method Using Near-Infrared Fluorescence and Development of a Detection Device. 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sapporo, March 2016.

40. Almasul Alfi, Bo Zhu, Jasmina Damnjanovic, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano: In Vitro Maturation of Soluble Manganese Peroxidase Expressed in Chaperone-Overexpressing E. coli. 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sapporo, March 2016.

41. Takaaki Kojima, Emi Kunitake, Kunio Ihara, Tetsuo Kobayashi, Hideo Nakano: Genome-Wide Analysis System for Filamentous-Fungal Transcriptional Regulatory Networks Using High-Speed DNA Sequencing. 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sapporo, March 2016.

42. Teruyo Ojima-Kato, Satomi Nagai, Takaaki Kojima, Hideo Nakano: Development of a High-Speed Screening Method for Rabbit Monoclonal Antibodies Using an Escherichia coli Cell-Free Protein Synthesis System. 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sapporo, March 2016.

2014

1. J. Damnjanovic, C. Kuroiwa, K. Ishida, H. Tanaka, H. Nakano, Y. Iwasaki: Engineering Phospholipase D for Stereoselective Conversion of Phosphatidylcholine into Bioactive 1-Phosphatidylinositol. 71st Semi-Annual Meeting of the Japanese Society of Enzyme Engineering, Fukuoka, April 2014.

2. Bo Zhu, Takuro Mizoguchi, Takaaki Kojima, Hideo Nakano: Cell-Free Synthesis of Horseradish Peroxidase and Single-Chain Lambda Cro Repressor Fusion Protein for Bead Display-Based High-Throughput Screening. 71st Semi-Annual Meeting of the Japanese Society of Enzyme Engineering, Fukuoka, April 2014.

3. Yugo Iwasaki, Jasmina Damnjanovic, Hideo Nakano: Enhancing the Thermostability of Phosphatidylinositol-Synthesizing Phospholipase D. 105th AOCS Annual Meeting and Expo, San Antonio, USA, May 2014.

4. Yugo Iwasaki: Protein-Engineering Modification of Microbial Phospholipases. 2014 Joint Seminar of the Oils and Fats Industrial Technology Division and Oleo Life Science Division, Tokyo, June 2014.

5. Kansuke Fukui, Hiroaki Yamamoto, Dai Hashimura, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Improvement of Antibody Expression Using a Cell-Free Protein Synthesis System. Summer Seminar 2014 for Young Researchers of the Society for Biotechnology, Japan, Kobe, July 2014.

6. Eri Ota, Bo Zhu, Ryoko Ninomiya, Takaaki Kojima, Hideo Nakano: Development of Enzyme-Engineering Technology for Manganese Peroxidase Using the Bead Display Method. Summer Seminar 2014 for Young Researchers of the Society for Biotechnology, Japan, Kobe, July 2014.

7. Yugo Iwasaki: Engineering of Streptomyces Phospholipase D and Its Application to Phospholipid Synthesis. Tokai Branch Oleochemistry Seminar, Japan Oil Chemists’ Society, Nagoya, June 2014.

8. Yugo Iwasaki: Establishment of a Loop-Trimming Method for Thermostabilization of Enzymes and Its Application to Phospholipase D. Asahi Glass Foundation 2014 Research Grant Presentation Meeting, Tokyo, July 2014.

9. Bo Zhu, Takuro Mizoguchi, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano: Cell-Free Synthesis of Horseradish Peroxidase and Single-Chain Lambda Cro Repressor Fusion Protein for Bead Display-Based High-Throughput Screening. 7th International Congress on Biocatalysis 2014, Hamburg, Germany, August 2014.

10. Takaaki Kojima, Hideo Nakano: High-Throughput Screening System for DNA Derived from Single Molecules Using the Bead Display Method. 66th Annual Meeting of the Society for Biotechnology, Japan, Sapporo, September 2014.

11. Kansuke Fukui, Hiroaki Yamamoto, Dai Hashimura, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Effect of Leucine-Zipper Addition on Fab Expression Using a Cell-Free Protein Synthesis System. 66th Annual Meeting of the Society for Biotechnology, Japan, Sapporo, September 2014.

12. Eri Ota, Bo Zhu, Ryoko Ninomiya, Takaaki Kojima, Hideo Nakano: Development of a High-Throughput Screening Method for Manganese Peroxidase Using the Bead Display Method. 66th Annual Meeting of the Society for Biotechnology, Japan, Sapporo, September 2014.

13. Bo Zhu, Takuro Mizoguchi, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano: Cell-Free Synthesis of Horseradish Peroxidase and Single-Chain Lambda Cro Repressor Fusion Protein for Bead Display-Based High-Throughput Screening. 66th Annual Meeting of the Society for Biotechnology, Japan, Sapporo, September 2014.

14. Marsel Murzabaev, Takaaki Kojima, Isao Kobayashi, Hideo Nakano: A Simple Flow-Focusing Device for High-Throughput Applications in Emulsion. 66th Annual Meeting of the Society for Biotechnology, Japan, Sapporo, September 2014.

15. Yugo Iwasaki, Jasmina Damnjanovic, Hideo Nakano: Development of Phospholipase D Having Phosphatidylinositol-Synthesizing Activity. 1st Asian Conference on Oleo Science, Sapporo, September 2014.

16. Yugo Iwasaki: Development of an sn-2-Position-Specific Lipase by Protein Engineering. Novozymes Bioforum 2014, Makuhari, October 2014.

17. Ayana Ujiie, Hideo Nakano, Yugo Iwasaki: Extracellular Secretory Expression of Candida antarctica Lipase B by Recombinant Escherichia coli. 171st Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2014.

18. Takuro Mizoguchi, Takaaki Kojima, Kiyotaka Hitomi, Hideo Nakano: Construction of a Novel Protein Bead Display Method Using a DNA-Binding Protein and Its Application to a Transglutaminase Assay. 171st Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2014.

19. Tomoya Okada, Weera Piyatheerawong, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano: Isolation and Identification of Cellulose-Degrading Thermophilic Bacteria from Compost and Analysis of Accumulated Hydrophobic Substances. 171st Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2014.

20. Bo Zhu, Takuro Mizoguchi, Takaaki Kojima, Hideo Nakano: In Vitro Synthesis of Horseradish Peroxidase with a DNA-Binding Tag and Its Fluorogenic Assay for Bead Display-Based Ultra-High-Throughput Screening. 171st Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2014.

21. Eri Ota, Bo Zhu, Takaaki Kojima, Hideo Nakano: Development of a High-Throughput Screening System for Filamentous-Fungal Manganese Peroxidase Using a DNA-Binding-Protein-Based Bead Display Method. 171st Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2014.

22. Chisato Kuroiwa, Ken Ishida, Hidetoshi Tanaka, Hideo Nakano, Yugo Iwasaki: Optimization of Regiospecific Phosphatidylinositol Synthesis by Protein Engineering of Phospholipase D. 171st Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2014.

23. Kansuke Fukui, Hiroaki Yamamoto, Dai Hashimura, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Improvement of Fab Antibody Expression by Addition of a Leucine Zipper. 171st Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2014.

24. Hideo Nakano, Eri Ota, Bo Zhu, Takaaki Kojima: Bead Display of Heme Enzymes for Function-Based High-Throughput Screening. Japan-Italy Joint Symposium, Nara, November 2014.

25. Yugo Iwasaki, Jasmina Damnjanovic, Hideo Nakano: Protein Engineering of Phospholipase D for Phosphatidylinositol Synthesis. Japan-Italy Joint Symposium, Nara, November 2014.

26. Jasmina Damnjanovic, Chisato Kuroiwa, Michiko Muraki, Ken Ishida, Hidetoshi Tanaka, Hideo Nakano, Yugo Iwasaki: Engineering of a Phospholipase D for Positionally Specific and Efficient Enzymatic Synthesis of 1-Phosphatidylinositol. Japan-Italy Joint Symposium, Nara, November 2014.

27. Bo Zhu, Takuro Mizoguchi, Takaaki Kojima, Hideo Nakano: In Vitro Synthesis of Horseradish Peroxidase with DNA-Binding Tag and Its Fluorogenic Assay for Bead Display-Based Ultra-High-Throughput Screening. Japan-Italy Joint Symposium, Nara, November 2014.

28. J. Damnjanovic, C. Kuroiwa, M. Muraki, K. Ishida, H. Tanaka, H. Nakano, Y. Iwasaki: Directing Positional Specificity in Enzymatic Synthesis of 1-Phosphatidylinositol by Protein Engineering of a Phospholipase D. 13th China-Japan-Korea Joint Symposium on Enzyme Engineering, Jeju, Korea, November 2014. [Best Poster Award]

29. Takaaki Kojima, Hideo Nakano: Development of High-Throughput Engineering Technology for Functional Biomolecules Using the Bead Display Method. Artificial Recognition Molecule Research Meeting, Hayama, March 2015.

30. Marsel Murzabaev, Takuro Mizoguchi, Takaaki Kojima, Isao Kobayashi, Hideo Nakano: In Vitro Transcription/Translation in Emulsion Produced by a Simple Flow-Focusing Device. 249th ACS National Meeting & Exposition, Denver, March 2015.

31. Keisuke Honma, Teruyo Ojima-Kato, Takaaki Kojima, Hiroshi Ueda, Hideo Nakano: Study on Improving the Efficiency of Antibody Fusion Protein Expression Using an Escherichia coli Intracellular Expression System. 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Okayama, March 2015.

32. Kansuke Fukui, Hiroaki Yamamoto, Teruyo Ojima-Kato, Takaaki Kojima, Hideo Nakano: Improvement of Fab Synthesis by Addition of a Leucine Zipper Using Escherichia coli Cell-Free and Live-Cell Protein Expression Systems. 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Okayama, March 2015.

33. Teruyo Ojima-Kato, Dai Hashimura, Satomi Nagai, Takaaki Kojima, Hideo Nakano: Generation of Rabbit Monoclonal Antibodies Using Escherichia coli Live-Cell and Cell-Free Expression Systems. 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Okayama, March 2015.

34. Akihiro Mori, Shoichi Hara, Tomohiro Sugawara, Takaaki Kojima, Yugo Iwasaki, Yasumasa Kawarasaki, Takehiko Sahara, Satoru Ogiya, Hideo Nakano: Selection of a Secretion Signal for Phanerochaete chrysosporium Endoglucanase from a Yeast Signal-Peptide Library. 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Okayama, March 2015.

35. Ayana Ujiie, Hideo Nakano, Yugo Iwasaki: Construction of an Extracellular Secretory Expression System for Candida antarctica Lipase B Using Recombinant Escherichia coli. 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Okayama, March 2015.

2013

1. Yugo Iwasaki: Protein-Engineering Modification of the Function of Streptomyces Phospholipase D. 69th Meeting of the Japanese Society of Enzyme Engineering, Nagoya, April 2013.

2. Jasmina Damnjanovic: Deletion of a Dynamic Loop as a Rational Strategy to Improve Stability of Phospholipase D. 2013 Chubu Branch Meeting of the Society for Biotechnology, Japan, Nagoya, August 2013.

3. Hideo Nakano, Takaaki Kojima: DNA Display Technology on Microbeads Using Emulsion PCR and Its Applications. 45th Autumn Meeting of the Society of Chemical Engineers, Japan, Okayama, September 2013.

4. Takuro Mizoguchi, Marsel Murzabaev, Isao Kobayashi, Takaaki Kojima, Kiyotaka Hitomi, Hideo Nakano: Development of a Novel Protein Bead Display Method Using a DNA-Binding Protein. 65th Annual Meeting of the Society for Biotechnology, Japan, Hiroshima, September 2013.

5. Aoi Okano, Ayana Ujiie, Hideo Nakano, Yugo Iwasaki: Secretory Expression of Heterologous Proteins by Recombinant Escherichia coli Using Streptomyces Phospholipase A2. 65th Annual Meeting of the Society for Biotechnology, Japan, Hiroshima, September 2013.

6. Tomoya Okada, Weera Piyatheerawong, Shoichi Hara, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano: Screening for Cellulose-Degrading Thermophilic Bacteria from Compost. 65th Annual Meeting of the Society for Biotechnology, Japan, Hiroshima, September 2013.

7. Chisato Kuroiwa, Ken Ishida, Hidetoshi Tanaka, Hideo Nakano, Yugo Iwasaki: Improvement of 1-PI Selectivity of Phosphatidylinositol-Synthesizing Phospholipase D. 65th Annual Meeting of the Society for Biotechnology, Japan, Hiroshima, September 2013.

8. Takaaki Kojima, Toshifumi Ezaki, Panhui Wang, Kyoko Kanamaru, Tetsuo Kobayashi, Hideo Nakano: Genome-Wide Screening of Binding Sites for the Filamentous-Fungal Transcription Factor AmyR Using the Bead Display Method. 65th Annual Meeting of the Society for Biotechnology, Japan, Hiroshima, September 2013.

9. Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Gate-Forming Loops Keep the Substrate Bound Inside the Active Site of Streptomyces Phospholipase D. 65th Annual Meeting of the Society for Biotechnology, Japan, Hiroshima, September 2013.

10. Hideo Nakano: Display of Macromolecules on Microbeads: A New Platform for Various Screening Methods. Enzyme Engineering XXII: Emerging Topics in Enzyme Engineering, Toyama, September 2013.

11. Jasmina Damnjanovic: Substrate-Binding Residues in Streptomyces Phospholipase D: Insights from Crystal Structures, Substrate Docking and Experimental Data. Enzyme Engineering XXII: Emerging Topics in Enzyme Engineering, Toyama, September 2013.

12. Takuro Mizoguchi, Marsel Murzabaev, Isao Kobayashi, Takaaki Kojima, Kiyotaka Hitomi, Hideo Nakano: Development of a Novel Protein Bead Display Method Using a DNA-Binding Protein and Its Application to a Transglutaminase Assay. 168th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2013.

13. Bo Zhu, Ryoko Ninomiya, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano: Cell-Free Synthesis of Horseradish Peroxidase and Manganese Peroxidase for Bead Display-Based High-Throughput Screening. 168th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2013.

14. Tomoya Okada, Weera Piyatheerawong, Shoichi Hara, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano: Isolation, Identification, and Characterization of Cellulose-Degrading Thermophilic Bacteria from Compost. 168th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2013.

15. Aoi Okano, Hideo Nakano, Yugo Iwasaki: Secretory Expression and Characterization of a Phospholipase A2-Single-Chain Antibody Fusion Protein by Recombinant Escherichia coli. 168th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2013.

16. Chisato Kuroiwa, Ken Ishida, Hidetoshi Tanaka, Hideo Nakano, Yugo Iwasaki: Improvement of 1-PI Specificity of Phospholipase D with Phosphatidylinositol-Synthesizing Activity. 168th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2013.

17. Marsel Murzabaev, Takaaki Kojima, Isao Kobayashi, Hideo Nakano: Uniform Emulsion for Biochemical Applications Using an Inexpensive Flow-Focusing Device. 168th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2013.

18. Teruyo Ojima-Kato, Dai Hashimura, Hiroaki Yamamoto, Takaaki Kojima, Hideo Nakano: Development of an Efficient Method for Obtaining Rabbit Monoclonal Antibodies. 2014 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Tokyo, March 2014.

2012

1. H. Nakano, P.W. Wang, T. Kojima: Comprehensive Analysis of Transcription Factor Binding Sites Using Bead Display of DNA. 12th Japan-China-Korea Joint Symposium on Enzyme Engineering, Kanazawa, May 2012.

2. B. Zhu, S. Mori, H. Matsuda, T. Kojima, H. Nakano: Development of Bead Display System for High-Throughput Screening of Cellulases Using Emulsion PCR and Cell-Free Protein Synthesis System. 12th Japan-China-Korea Joint Symposium on Enzyme Engineering, Kanazawa, May 2012.

3. Y. Ito, T. Kojima, S. Ohuchi, H. Nakano: In Vitro High-Throughput Promoter Screening System Using a Bead Display and a Class I Ligase Ribozyme. 12th Japan-China-Korea Joint Symposium on Enzyme Engineering, Kanazawa, May 2012.

4. J. Damnjanovic, H. Nakano, Y. Iwasaki: Characterization of Streptomyces antibioticus Phospholipase D Deletion Mutant. 12th Japan-China-Korea Joint Symposium on Enzyme Engineering, Kanazawa, May 2012.

5. Yugo Iwasaki: Alteration of the Substrate Specificity of Streptomyces Phospholipase D. Enzyme and Coenzyme Research Meeting, Nagoya, July 2012.

6. J. Damnjanovic, H. Nakano, Y. Iwasaki: Synthesis of Phosphatidylinositol Catalyzed by Thermostable Variants of Streptomyces Phospholipase D. 15th European Congress of Biotechnology, Istanbul, Turkey, September 2012.

7. J. Damnjanovic, H. Nakano, Y. Iwasaki: Surface Loop Deletion as a Strategy for Enzyme Thermostabilization. 64th Annual Meeting of the Society for Biotechnology, Japan, Kobe, October 2012.

8. Ken Ishida, Hidetoshi Tanaka, Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Amino-Acid Residues Determining Inositol Orientation in Phosphatidylinositol-Synthesizing Phospholipase D. 64th Annual Meeting of the Society for Biotechnology, Japan, Kobe, October 2012.

9. Ryoko Ninomiya, Bo Zhu, Isao Kobayashi, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano: Construction of a High-Throughput Screening System for Filamentous-Fungal Lignin-Degrading Enzymes Using the Bead Display Method. 64th Annual Meeting of the Society for Biotechnology, Japan, Kobe, October 2012.

10. Tomoaki Eba, Hideo Nakano, Yugo Iwasaki: Effect of the sn-3 Position of the Substrate on the Reactivity of Streptomyces Phospholipase A2. 165th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2012.

11. Ken Ishida, Hidetoshi Tanaka, Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Amino-Acid Residues Determining the Orientation of Inositol in Phosphatidylinositol-Synthesizing Phospholipase D. 165th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2012.

12. Ryoko Ninomiya, Bo Zhu, Isao Kobayashi, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano: Construction of a High-Throughput Screening System for Filamentous-Fungal Lignin-Degrading Enzymes Using the Bead Display Method. 165th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2012.

13. Shoichi Hara, Tomohiro Sugawara, Takehiko Sahara, Satoru Ogiya, Yasumasa Kawarasaki, Takaaki Kojima, Hideo Nakano: Development of a Signal-Peptide Optimization Tool Using a Yeast Signal-Peptide Library. 165th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2012.

14. Yurie Ito, Takaaki Kojima, Masashi Ohuchi, Hideo Nakano: Establishment of an In Vitro High-Throughput Promoter Screening Method Using the Bead Display Method. 165th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2012.

15. Toshifumi Ezaki, Panhui Wang, Takaaki Kojima, Hideo Nakano: Detection System for DNA-Filamentous Fungal Transcription Factor Interactions Using the Bead Display Method. 165th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2012.

16. Yugo Iwasaki: Improvement of the Positional Specificity of Phosphatidylinositol-Synthesizing Phospholipase D. Research Presentation Meeting of the Japan Association for Applied Enzymology, Osaka, November 2012.

17. Yugo Iwasaki: Synthesis of Phosphatidylinositol Using Engineered Phospholipase D. 11th Phosphatidylserine Research Meeting, Tokyo, November 2012.

18. B. Zhu, T. Kojima, Y. Iwasaki, H. Nakano: Cell-Free Synthesis of Horseradish Peroxidase C for Bead Display-Based High-Throughput Screening. 2013 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Sendai, March 2013.

2011

1. Takaaki Kojima, Yurie Ito, Masashi Ohuchi, Hideo Nakano: In Vitro Selection of Promoter Activity Using the Bead Display Method and a Ligase Ribozyme. Summer Seminar 2011 for Young Researchers of the Society for Biotechnology, Japan, Fuefuki, July 2011.

2. T. Kojima, Y. Ito, S. Ohuchi, H. Nakano: Novel Screening Method for Promoter Activity Using a Bead Display and a Ligase Ribozyme. 5th GCOE Retreat, Nagahama, September 2011.

3. P. Wang, T. Kojima, H. Nakano: Detection of DNA-Transcription Factor Interactions Using a Microbead Display System. 5th GCOE Retreat, Nagahama, September 2011.

4. D. Takemori, K. Yoshino, H. Nakano, Y. Iwasaki: Extracellular Production of Active Phospholipase A2 from Streptomyces violaceoruber by Recombinant Escherichia coli. 5th GCOE Retreat, Nagahama, September 2011.

5. J. Damnjanovic, R. Takahashi, H. Nakano, Y. Iwasaki: Phosphatidylinositol (PI) Synthesis Using Thermostable Streptomyces antibioticus Phospholipase D (PLD) Mutants. International Union of Microbiological Societies 2011 Congress, Sapporo, September 2011.

6. J. Damnjanovic, R. Takahashi, H. Nakano, Y. Iwasaki: A Rapid and Efficient HPLC Method for Profiling Phospholipids from Soy Lecithin Used as Substrate for 1(3)-PI Synthesis Catalyzed by Mutant Phospholipase D. 9th Euro Fed Lipid Congress, Amsterdam, September 2011.

7. Takaaki Kojima, Yurie Ito, Masashi Ohuchi, Hideo Nakano: In Vitro High-Throughput Screening of Promoter Activity Using the Bead Display Method and a Class I Ligase Ribozyme. 63rd Annual Meeting of the Society for Biotechnology, Japan, Koganei, September 2011.

8. Yugo Iwasaki: Enzymatic Synthesis of Phosphatidylinositols by Phospholipase D. 7th International Symposium on Biocatalysis and Agricultural Biotechnology, Kyoto, October 2011.

9. Ayataka Okuma, Hideo Nakano, Yugo Iwasaki: Construction of an Escherichia coli Expression System for Porcine Pancreatic Phospholipase A2. 2011 Joint Meeting of the Kansai and Chubu Branches, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, October 2011.

10. Daiki Takemori, Kenta Yoshino, Tomoaki Eba, Hideo Nakano, Yugo Iwasaki: Production of Streptomyces-Derived Phospholipase A2 Using Escherichia coli. 2011 Joint Meeting of the Kansai and Chubu Branches, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, October 2011.

11. Hideo Nakano, Takaaki Kojima: High-Throughput Screening System for Molecular Interactions Using Emulsion PCR. 2011 Biotechnology Technology Seminar, “Latest Screening Technologies for Drug Discovery,” Tokyo, November 2011.

12. Yugo Iwasaki: Enzymatic Synthesis of Phosphatidylinositol Using Engineered Phospholipase D. Interdisciplinary Lipid Creation Research Division, Osaka, January 2011.

13. Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki: Influence of Surface Loop (D40 Loop) Deletion on Expression and Stability of Streptomyces antibioticus Phospholipase D. 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto Women’s University, March 2012.

14. Bo Zhu, Shoya Mori, Hideki Matsuda, Takaaki Kojima, Hideo Nakano: Development of Bead Display System for Molecular Evolution of Cellulases. 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2012.

15. Yurie Ito, Takaaki Kojima, Masashi Ohuchi, Hideo Nakano: Establishment of a High-Throughput In Vitro Screening Method Using the Bead Display Method and a Class I Ligase Ribozyme. 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2012.

16. Panhui Wang, Takaaki Kojima, Hideo Nakano: Study of a DNA-Transcription Factor Interaction Detection System Using Bead Display. 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2012.

17. Shoichi Hara, Tomohiro Sugawara, Takehiko Sahara, Satoru Ogiya, Yasumasa Kawarasaki, Takaaki Kojima, Hideo Nakano: Development of a Signal-Peptide Optimization Tool Using a Yeast Signal-Peptide Library. 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2012.

18. Hideo Nakano, Takaaki Kojima: Development and Application of Library-Construction Technologies Using a Cell-Free Protein Synthesis System. 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2012.

2010

1. Hideo Nakano: Bead Display: A High-Throughput Screening Technology of Functional Proteins and DNAs. 2010 International Symposium & Annual Meeting of the Korean Society for Microbiology and Biotechnology, Seoul, Korea, June 2010.

2. Yunita Sabrina: High-Throughput Monoclonal Antibody Production Using Single-Cell RT-PCR and Cell-Free Protein Synthesis System. 2010 Chubu Branch Meeting of the Society for Biotechnology, Japan, Nagoya, August 2010.

3. Takaaki Kojima, Yoko Hashimoto, Masashi Kato, Tetsuo Kobayashi, Hideo Nakano: High-Throughput Screening of DNA-Binding Sites for the Fungal Transcription Factor AmyR Using a Bead Display System of DNA. 4th Global COE Retreat, Toyohashi, September 2010.

4. Panhui Wang, Takaaki Kojima, Hideo Nakano: Detection of DNA-Transcription Factor Interactions Using a Microbead Display System. 4th Global COE Retreat, Toyohashi, September 2010.

5. Hideo Nakano, Takaaki Kojima: Technology for Analyzing mRNA Derived from Single Molecules and Single Cells Using a Cell-Free Protein Synthesis System. Annual Meeting of the Society for Biotechnology, Japan, Miyazaki, October 2010.

6. Yumiko Mikami, Hideki Matsuda, Daigo Matsui, Takaaki Kojima, Hideo Nakano: Stabilization of DNA-Protein Complexes in the Bead Display Method. 62nd Annual Meeting of the Society for Biotechnology, Japan, Miyazaki, October 2010.

7. Ryota Hara, Sho Yahata, Takaaki Kojima, Hideo Nakano: Isolation of Anti-Influenza Virus Monoclonal Antibodies Using Single-Cell RT-PCR and a Cell-Free Protein Synthesis System. 62nd Annual Meeting of the Society for Biotechnology, Japan, Miyazaki, October 2010.

8. Takaaki Kojima, Masashi Ohuchi, Hideo Nakano: Development of a Promoter-Activity Selection Method Using the Bead Display Method and a Ligase Ribozyme. 62nd Annual Meeting of the Society for Biotechnology, Japan, Miyazaki, October 2010.

9. Ken Sakai, Takaaki Kojima, Nao Yamakawa, Yuji Nishiura, Hiroshi Tanaka, Takashi Takahashi, Chihiro Sato, Ken Kitajima, Hideo Nakano: Analysis Method for Glycan-Binding Proteins Using a Cell-Free Protein Synthesis System and the Bead Display Method. 62nd Annual Meeting of the Society for Biotechnology, Japan, Miyazaki, October 2010.

10. Takashi Nagaya, Takaaki Kojima, Hideo Nakano: Establishment of a Novel Activity Detection Method for the Tyrosine Kinase Csk Using the Bead Display Method. 159th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2010.

11. Ryota Hara, Sho Yahata, Takaaki Kojima, Hideo Nakano: Development of a Novel Method for Obtaining Anti-Influenza Virus Monoclonal Antibodies Using a Cell-Free Protein Synthesis System and Single-Cell RT-PCR. 159th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2010.

12. Kazuhiro Nagasaka, Yugo Iwasaki, Hideo Nakano: Selective Synthesis of Phosphatidylinositol Isomers Using Phospholipases. 159th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2010.

13. Daiki Takemori, Kenta Yoshino, Yugo Iwasaki, Hideo Nakano: Construction of an Escherichia coli Expression System for Streptomyces Phospholipase A2. 159th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2010.

14. Shoya Mori, Hideki Matsuda, Takaaki Kojima, Hideo Nakano: Development of a Novel Flow-Cytometric Method for Detecting Cellulase Activity Using the Bead Display Method. 159th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2010.

15. Akari Ozaki, Yugo Iwasaki, Hideo Nakano: Analysis of the Regioselectivity of Phosphatidylinositol-Synthesizing Phospholipase D. 159th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2010.

16. Yumiko Mikami, Hideki Matsuda, Daigo Matsui, Takaaki Kojima, Hideo Nakano: Establishment of a Method for Stabilizing DNA-Protein Complexes in the Bead Display Method Using Glutaraldehyde. 159th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2010.

17. Ken Sakai, Takaaki Kojima, Nao Yamakawa, Yuji Nishiura, Hiroshi Tanaka, Takashi Takahashi, Chihiro Sato, Ken Kitajima, Hideo Nakano: A Novel Method for Analyzing Glycan-Binding Proteins Using Microbead Display and a Cell-Free Protein Synthesis System. 159th Meeting of the Chubu Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Nagoya, October 2010.

18. Takaaki Kojima, Hideo Nakano: Bead Display: A Novel High-Throughput Screening Technology to Explore Functional Biomolecules. 11th China-Japan-Korea Joint Symposium on Enzyme Engineering, Chengdu, China, November 2010.

19. Yugo Iwasaki: Streptomyces Phospholipase D with Altered Substrate Specificity Capable of Synthesizing Phosphatidylinositols. 8th Euro Fed Lipid Congress, Munich, Germany, November 2010.

20. Yugo Iwasaki, Atsushi Masayama, Hideo Nakano: Altering Substrate Specificity of Phospholipase D by Protein Engineering. 2010 International Chemical Congress of Pacific Basin Societies, Honolulu, Hawaii, USA, December 2010.

21. Daiki Takemori, Kenta Yoshino, Hideo Nakano, Yugo Iwasaki: Extracellular Expression of Active Phospholipase A2 by Recombinant Escherichia coli. 2010 International Chemical Congress of Pacific Basin Societies, Honolulu, Hawaii, USA, December 2010.

22. Daiki Takemori, Kenta Yoshino, Hideo Nakano, Yugo Iwasaki: Construction of an Escherichia coli Expression System for Streptomyces Phospholipase A2. 2011 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2011. [Presentation Cancelled Due to the Great East Japan Earthquake]

23. Panhui Wang, Takaaki Kojima, Hideo Nakano: High-Throughput Detection of DNA-Filamentous Fungal Transcription Factor Interactions Using the DNA Bead Display Method. 2011 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2011. [Presentation Cancelled Due to the Great East Japan Earthquake]

24. Shoya Mori, Yumiko Mikami, Hideki Matsuda, Takaaki Kojima, Hideo Nakano: High-Throughput Analysis of Cellulase Activity Using the Bead Display Method. 2011 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2011. [Presentation Cancelled Due to the Great East Japan Earthquake]

25. Takaaki Kojima, Takashi Nagaya, Hideo Nakano: High-Throughput Screening of Tyrosine Kinase Substrate Peptides Using the Bead Display Method. 2011 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kyoto, March 2011. [Presentation Cancelled Due to the Great East Japan Earthquake]